Coding

Part:BBa_K4998018:Design

Designed by: Rafail Andreou   Group: iGEM23_thessaloniki   (2023-10-09)


Laccase gene (silA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 326
    Illegal XhoI site found at 199
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 130
    Illegal NgoMIV site found at 792
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 76


Design Notes

In order for the enzyme to fuction extracellularly, a signal peptide from the Sec or Tat pathways should be encoded on the laccase's N-terminus.


Source

Streptomyces ipomonae

References